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Recombinant Human CD154/CD40L/TNFSF5 Protein

Protein

Recombinant Human CD154/CD40L/TNFSF5 Protein

  • Expression Host: HEK293 Cells
  • Species: Human
  • Size: 50ug,100ug,500ug
  • Official Symbol: CD154

CD40LG (CD40 Ligand) is a Protein Coding gene. Diseases associated with CD40LG include Immunodeficiency With Hyper-Igm, Type 1 and Cd40 Ligand Deficiency. Among its related pathways are MIF Mediated Glucocorticoid Regulation and ERK Signaling. Gene Ontology (GO) annotations related to this gene include cytokine activity and CD40 receptor binding. An important paralog of this gene is TNFSF10.Cytokine that acts as a ligand to CD40/TNFRSF5.Costimulates T-cell proliferation and cytokine production.Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation.  

 

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Product Background

CD40 Ligand(CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.

Product Specification

Catalog Number

ALP64645

Product Name

Recombinant Human CD154/CD40L/TNFSF5 Protein

Alias

Tumor Necrosis Factor Ligand Superfamily Member 5, CD154

Alternate Names

cd154 gene,cd154,cd40l,cd40l protein,recombinant cd40l,human cd40l,cd40l gene,Recombinant Protein,cd154 protein,Human CD40L Protein

Official Symbol

CD154/CD40L/TNFSF5

Species

Human

Expression Host

HEK293 Cells

Protein Family

TNF Superfamily

Product Description

ALP64645 Recombinant Human CD40L Protein is produced in HEK293 Cells and the target gene encoding Met113-Leu261 is expressed.

Size

50ug,100ug,500ug

Purification

Affinity Purification

Purity

>90%-95% as determined by SDS-PAGE

Endotoxin

<1.0 EU/ug determined by the LAL method

Buffer

20mM PB, 200mM NaCl, 0.1mM EDTA, pH7.0

Storage

For long term storage, store the reagent at -80°C. Avoid repeated freeze-thaw cycles.

Shipping Condition

Lyophilized powder is shipped at ambient temperature. Liquid form is shipped in dry ice.

Note

This product is for research use only. This product can not be used for human.

Protein Pathways

Allograft rejection, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Cytokine-cytokine receptor interaction, Primary immunodeficiency, Systemic lupus erythematosus, T cell receptor signaling pathway, Viral myocarditis

Reference

1 Murphy, K. M., & Reiner, S. L., Nature Reviews Immunology, 2003, DOI: 10.1038/nri1045

2 Fuleihan, R. L., et al., Journal of Pediatrics, 1997, DOI: 10.1016/S0022-3476(97)80445-8

3 Aste-Amezaga, M., et al., Journal of Immunology, 2000, DOI: 10.4049/jimmunol.164.8.4190

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