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Recombinant Human CD28 Protein, His Tag

Protein

Recombinant Human CD28 Protein, His Tag

  • Expression Host: HEK293 Cells
  • Species: Human
  • Size: 50ug,100ug,500ug
  • Official Symbol: CD28

CD28 (CD28 Molecule) is a Protein Coding gene. Diseases associated with CD28 include Classic Mycosis Fungoides and Sezary's Disease. Among its related pathways are CD28 co-stimulation and PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling. Gene Ontology (GO) annotations related to this gene include identical protein binding and obsolete SH3/SH2 adaptor activity. An important paralog of this gene is CTLA4.Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation.Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells.

 

Alpha Lifetech specializes in producing high-quality recombinant proteins to ensure purity and activity for various research applications.

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Product Background

T-cell-specific surface glycoprotein CD28(CD28) is a single-pass typeI membrane protein which contains one Ig-likeV-type(immunoglobulin-like) domain. It belongs to the immunoglobulin(Ig) superfamily. CD28 is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation.CD28 co-stimulation is necessary for CD4 positive T-cell proliferation and survival, interleukin-2 production, and T-helper type-2 development. Human post-thymic regulatory T cells require CD28 co-stimulation to expand and maintain potent suppressive function in vivo. Apoptosis plays a key role in the age-related decline of CD28 expression and in immunosenescence. CD28 is the receptor for CD80(B7.1) and CD86(B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells(APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Product Specification

Catalog Number

ALP64537

Product Name

Recombinant Human CD28 Protein, His Tag

Alias

CD28 molecule, Tp44

Alternate Names

cd28,cd28 gene,cd28 marker,cd28 protein,cd28 signaling,recombinant cd28,recombinant cd28 protein,human cd28,human cd28 protein,recombinant human cd28

Official Symbol

CD28

Species

Human

Expression Host

HEK293 Cells

Protein Family

Immunoglobulin Superfamily

Product Description

ALP64537 Recombinant Human CD28 Protein, His Tag is produced in HEK293 Cells and the target gene encoding Asn19-Pro152 is expressed with a 6His tag at the N-terminus.

Size

50ug,100ug,500ug

Purification

Affinity Purification

Purity

>90%-95% as determined by SDS-PAGE

Endotoxin

<1.0 EU/ug determined by the LAL method

Buffer

20mM Tris, 150mM NaCl, pH8.0

Storage

For long term storage, store the reagent at -80°C. Avoid repeated freeze-thaw cycles.

Shipping Condition

Lyophilized powder is shipped at ambient temperature. Liquid form is shipped in dry ice.

Note

This product is for research use only. This product can not be used for human.

Reference

1 Acuto, O., Michel, F., Nature Reviews Immunology, 2003, DOI: 10.1038/nri1260

2 Linsley, P. S., Ledbetter, J. A., Annual Review of Immunology, 1993, DOI: 10.1146/annurev.iy.11.040193.001343

3 Salomon, B., Bluestone, J. A., Annual Review of Immunology, 2001, DOI: 10.1146/annurev.immunol.19.1.225

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