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Recombinant Human CD73 Protein, Fc Tag

Protein

Recombinant Human CD73 Protein, Fc Tag

  • Expression Host: HEK293 Cells
  • Species: Human
  • Size: 50ug,100ug,500ug
  • Official Symbol: CD73

NT5E (5'-Nucleotidase Ecto) is a Protein Coding gene. Diseases associated with NT5E include Calcification Of Joints And Arteries and Periarthritis. Among its related pathways are Metabolism of nucleotides and Infectious disease. Gene Ontology (GO) annotations related to this gene include nucleotide binding and hydrolase activity, acting on ester bonds.Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate.Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms.Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN.

Alpha Lifetech can provide Recombinant Protein products. Including various specifications such as 50, 100, and 500ug, this protein product has been purified and passed quality inspection. Scientists from all over the world are welcome to consult.

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Product Background

CD73 is a glycosyl phosphatidylinositol(GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries(CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.

Product Specification

Catalog Number

ALP64515

Product Name

Recombinant Human CD73 Protein, Fc Tag

Alias

5'-Nucleotidase, NT5E

Alternate Names

cd73,cd73 inhibitor,cd73 gene,cd73 marker,cd73 expression,human cd73 elisa,Human CD73 Protein,fc tag,fc tag protein,Recombinant Protein

Official Symbol

CD73

Species

Human

Expression Host

HEK293 Cells

Protein Family

5'-nucleotidase Family

Product Description

ALP64515 Recombinant Human CD73 Protein, Fc Tag is produced in HEK293 Cells and the target gene encoding Trp27-Lys547 is expressed with a Fc tag at the C-terminus.

Size

50ug,100ug,500ug

Purification

Affinity Purification

Purity

>90%-95% as determined by SDS-PAGE

Endotoxin

<1.0 EU/ug determined by the LAL method

Buffer

20mM TrisHCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH7.5

Storage

For long term storage, store the reagent at -80°C. Avoid repeated freeze-thaw cycles.

Shipping Condition

Lyophilized powder is shipped at ambient temperature. Liquid form is shipped in dry ice.

Note

This product is for research use only. This product can not be used for human.

Protein Pathways

Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism

Reference

1 Allard, B., Longhi, M. S., Robson, S. C., & Stagg, J., Immunological Reviews, 2017, DOI: 10.1111/imr.12555

2 Stagg, J., & Smyth, M. J., Journal of Biological Chemistry, 2010, DOI: 10.1074/jbc.R110.161349

3 Junger, W. G., Nature Reviews Immunology, 2011, DOI: 10.1038/nri2938

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