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Recombinant Human ILT3/CD85k/LILRB4 Protein, His Tag

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Recombinant Human ILT3/CD85k/LILRB4 Protein, His Tag

  • Expression Host: HEK293 Cells
  • Species: Human
  • Size: 50ug,100ug,500ug
  • Official Symbol: ILT3

LILRB4 (Leukocyte Immunoglobulin Like Receptor B4) is a Protein Coding gene. Among its related pathways are Innate Immune System and Class I MHC mediated antigen processing and presentation. Gene Ontology (GO) annotations related to this gene include signaling receptor activity and antigen binding. An important paralog of this gene is LILRB3.Inhibitory receptor involved in the down-regulation of the immune response and the development of immune tolerance.Receptor for FN1.Receptor for apolipoprotein APOE.

 

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Product Background

Mouse Leukocyte Immunoglobulin-like Receptor Subfamily B Member 4(LILRB4/CD85k/ILT3) is an approximately transmembrane glycoprotein that negatively regulates immune cell activation. Mouse LILRB4 consists of a 215 amino acid(aa) extracellular domain with two Ig-like domains, a 22 aa transmembrane segment, and a 75 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs(ITIM). Within the ECD, mouse LILRB4 shares 45% and 77% aa sequence identity with human and rat LILRB4, respectively. Alternative splicing of mouse LILRB4 generates a potentially soluble isoform that lacks the transmembrane segment. LILRB4 is expressed on dendritic cells(DC), monocytes, macrophages, and vascular endothelial cells(EC). Ligation of LILRB4 triggers ITIM-mediated inhibition of cellactivating signaling, leading to enhanced immune tolerance and reduced allogeneic graft rejection. Soluble LILRB4 induces the differentiation of CD8+ T suppressor cells(Ts) that can inhibit the effector functions of CD4+ Th cells and CD8+ CTL. In turn, CD8+ Ts cells induce LILRB4 up-regulation and a tolerogenic phenotype in monocytes, DC, and EC.

Product Specification

Catalog Number

ALP64550

Product Name

Recombinant Human ILT3/CD85k/LILRB4 Protein, His Tag

Alias

Leukocyte immunoglobulin-like receptor subfamily B member 4, ILT3

Alternate Names

ilt3,ilt3 protein,cd85k,lilrb4,Recombinant Protein,CD85K protein,lilrb4 protein,his tag,his tag protein,Human ILT3 Protein

Official Symbol

ILT3/CD85k/LILRB4

Species

Human

Expression Host

HEK293 Cells

Protein Family

LIP Family

Product Description

ALP64550 Recombinant Human ILT3 Protein, His Tag is produced in HEK293 Cells and the target gene encoding Gln22-Glu259 is expressed with a 6His tag at the C-terminus.

Size

50ug,100ug,500ug

Purification

Affinity Purification

Purity

>90%-95% as determined by SDS-PAGE

Endotoxin

<1.0 EU/ug determined by the LAL method

Buffer

Supplied in PBS, pH7.4

Storage

For long term storage, store the reagent at -80°C. Avoid repeated freeze-thaw cycles.

Shipping Condition

Lyophilized powder is shipped at ambient temperature. Liquid form is shipped in dry ice.

Note

This product is for research use only. This product can not be used for human.

Reference

1 Zhang, J., et al., Cancer Immunology, Immunotherapy, 2021, DOI: 10.1007/s00262-021-02956-8

2 Nakamura, K., & Dai, Z., Nature Reviews Cancer, 2020, DOI: 10.1038/s41571-020-00428-4

3 Jiang, Y., et al., Journal of Immunology, 2019, DOI: 10.4049/jimmunol.1901021

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