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Recombinant Human INDO/IDO/IDO1 Protein, His Tag

Protein

Recombinant Human INDO/IDO/IDO1 Protein, His Tag

  • Expression Host: E. coli
  • Species: Human
  • Size: 50ug,100ug,500ug
  • Official Symbol: INDO

IDO1 (Indoleamine 2,3-Dioxygenase 1) is a Protein Coding gene. Diseases associated with IDO1 include Listeriosis and Eosinophilia-Myalgia Syndrome. Among its related pathways are superpathway of tryptophan utilization and NAD metabolism. Gene Ontology (GO) annotations related to this gene include heme binding and tryptophan 2,3-dioxygenase activity.Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway.Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses.Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. 

 

Alpha Lifetech specializes in producing high-quality recombinant proteins to ensure purity and activity for various research applications.

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Product Background

Indoleamine 2,3-dioxygenase(IDO) is a heme enzyme that initiates the oxidative degradation of the least abundant, essential amino acid, l-tryptophan, along the kynurenine pathway. This protein is normally expressed in the dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. IDO activity is associated with immunosuppression and immune attenuation. Several studies showed that IDO can contribute to immune escape when expressed directly in tumor cells or when expressed in immunosuppressive antigen presenting cells such as tolerogenic dendritic cells or tumor associated macrophages. IDO also is a promising therapeutic target for the treatment of cancer, chronic viral infections, and other diseases characterized by pathological immune suppression.

Product Specification

Catalog Number

ALP64589

Product Name

Recombinant Human INDO/IDO/IDO1 Protein, His Tag

Alias

Indole 2, 3-dioxygenase, INDO

Alternate Names

ido1 gene,ido1 immune checkpoint,ido gene,ido protein,ido1 protein,Recombinant Protein,Human INDO Protein,his tag,his tag protein

Official Symbol

INDO/IDO/IDO1

Species

Human

Expression Host

E. coli

Product Description

ALP64589 Recombinant Human INDO Protein, His Tag is produced in E.coli and the target gene encoding Met1-Gly403 is expressed with a 6His tag at the N-terminus.

Size

50ug,100ug,500ug

Purification

Affinity Purification

Purity

>90%-95% as determined by SDS-PAGE

Endotoxin

<1.0 EU/ug determined by the LAL method

Buffer

20mM Sodium Acetate, 150mM NaCl and 20% Glycerol, pH4.5

Storage

For long term storage, store the reagent at -80°C. Avoid repeated freeze-thaw cycles.

Shipping Condition

Lyophilized powder is shipped at ambient temperature. Liquid form is shipped in dry ice.

Note

This product is for research use only. This product can not be used for human.

Protein Pathways

Metabolic pathways, Tryptophan metabolism

Reference

1 Mellor, A. L., & Munn, D. H., Nature Reviews Immunology, 2004, DOI: 10.1038/nri1177

2 Platten, M., Wick, W., & Van den Eynde, B. J., Cancer Research, 2012, DOI: 10.1158/0008-5472.CAN-11-3768

3 Munn, D. H., Sharma, M. D., Hou, D., Baban, B., Lee, J. R., Antonia, S. J., ... & Mellor, A. L., Journal of Clinical Investigation, 2004, DOI: 10.1172/JCI200421853

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