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Recombinant Mouse CD154/CD40L/TNFSF5 Protein, His Tag

Protein

Recombinant Mouse CD154/CD40L/TNFSF5 Protein, His Tag

  • Expression Host: HEK293 Cells
  • Species: Mouse
  • Size: 50ug,100ug,500ug
  • Official Symbol: CD154

CD40LG (CD40 Ligand) is a Protein Coding gene. Diseases associated with CD40LG include Immunodeficiency With Hyper-Igm, Type 1 and Cd40 Ligand Deficiency. Among its related pathways are MIF Mediated Glucocorticoid Regulation and ERK Signaling. Gene Ontology (GO) annotations related to this gene include cytokine activity and CD40 receptor binding. An important paralog of this gene is TNFSF10.Cytokine that acts as a ligand to CD40/TNFRSF5.Costimulates T-cell proliferation and cytokine production.Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation.  

 

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Product Background

CD40 Ligand, also known as TNFSF5, CD154, is a type II transmembrane glycoprotein member of the TNF superfamily. Mature mouse CD40 Ligand consists of a 22 amino acid(aa) cytoplasmic domain, a transmembrane segment, and a 214 aa extracellular region. CD40 Ligand is expressed as a homotrimer on platelets and activated T cells and B cells. It is up-regulated following stimulation of basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds and activates CD40, which is expressed on the surface of B cells, dendritic cells,macrophages, monocytes, platelets, endothelial cells, and epithelial cells. Monomeric, dimeric, and trimeric forms of soluble CD40 Ligand bind to oligomeric CD40 on cell membranes. CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses. CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS. It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis.

Product Specification

Catalog Number

ALP64699

Product Name

Recombinant Mouse CD154/CD40L/TNFSF5 Protein, His Tag 

Alias

Tumor Necrosis Factor Ligand Superfamily Member 5, CD154

Alternate Names

cd154 gene,cd154,cd40l,mouse cd40l,cd40l protein,recombinant cd40l,Mouse CD40L Protein,cd40l gene,Recombinant Protein,cd154 protein,his tag,his tag protein

Official Symbol

CD154/CD40L/TNFSF5

Species

Mouse

Expression Host

HEK293 Cells

Protein Family

TNF Superfamily

Product Description

ALP64699 Recombinant Mouse CD40L Protein, His Tag is produced in HEK293 Cells and the target gene encoding Met112-Leu260 is expressed with a 6His tag at the N-terminus.

Size

50ug,100ug,500ug

Purification

Affinity Purification

Purity

>90%-95% as determined by SDS-PAGE

Endotoxin

<1.0 EU/ug determined by the LAL method

Buffer

20mM PB, 200mM NaCl, 0.1mM EDTA, pH7.0

Storage

For long term storage, store the reagent at -80°C. Avoid repeated freeze-thaw cycles.

Shipping Condition

Lyophilized powder is shipped at ambient temperature. Liquid form is shipped in dry ice.

Note

This product is for research use only. This product can not be used for human.

Reference

1 Holt, P. G., & Jones, C. A., Immunological Reviews, 2005, DOI: 10.1111/j.1600-065X.2005.00200.x

2 Michels, A. W., & Eisenbarth, G. S., Nature Reviews Rheumatology, 2010, DOI: 10.1038/nrrheum.2010.36

3 Mao, Y., et al., Cancer Immunology, Immunotherapy, 2017, DOI: 10.1007/s00262-017-2032-3

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