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Recombinant Mouse CD279/PD-1 Protein, His Tag

Protein

Recombinant Mouse CD279/PD-1 Protein, His Tag

  • Expression Host: HEK293 Cells
  • Species: Mouse
  • Size: 50ug,100ug,500ug
  • Official Symbol: CD279

PDCD1 (Programmed Cell Death 1) is a Protein Coding gene. Diseases associated with PDCD1 include Systemic Lupus Erythematosus 2 and Multiple Sclerosis. Among its related pathways are Infectious disease and SARS-CoV-2 Infection. Gene Ontology (GO) annotations related to this gene include obsolete signal transducer activity.Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self.Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2.Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity).

 

Scientists at Alpha Lifetech have expertise in protein engineering, and with extensive expertise in protein engineering, we provide optimized recombinant proteins that enhance stability and functionality.

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Product Background

Programmed Death-1(PD-1), firstly cloned from mouse T cell hybridoma 2B4.11, is one member of CD28/CTLA-4 superfamily. PD-1 belongs to type I transmembrane protein and acts as an important immunosuppressive molecule. The cytoplamsic tail of PD-1 contains two structural motifs, an immunoreceptor tyrosine-based inhibitory motif(ITIM) and an immunoreceptor tyrosine-based switch motif(ITSM) formed by two tyrosine residues which make the difference in PD-1 signal mediating. Mouse PD-1 is expressed in thymus and shares about 69% aa sequence identity with human PD-1. Recently, programmed death-1(PD-1) with its ligands, programmed death ligand B7H1(PD-L1) and B7DC(PD-L2), was found to regulate T-cell activation and tolerance, upon ligand binding, inhibiting T-cell effector functions in an antigen-specific manner. PD-1 gene knocked out mice would induce some autoimmune diseases, which suggests that PD-1 acts as a co-inhibitory molecule actively participating in maintaining peripheral tolerance. Thus, PD-1 may be a useful target for the immunologic therapy of carcinoma,infection,autoimmune diseases as well as organ transplantation.

Product Specification

Catalog Number

ALP64637

Product Name

Recombinant Mouse CD279/PD-1 Protein, His Tag

Alias

Programmed cell death protein 1

Alternate Names

cd279 gene,cd279 protein,mouse cd279,pd-1 inhibitor,pd-1 gene,pd-1 protein,recombinant pd-1,recombinant pd-1 protein,pd-1,pd-1 ligands,pd-1 checkpoint inhibitor,Recombinant Protein,Mouse CD279 Protein,his tag,his tag protein

Official Symbol

CD279/PD-1

Species

Mouse

Expression Host

HEK293 Cells

Protein Family

CTLA-4 Family

Product Description

ALP64637 Recombinant Mouse CD279 Protein, His Tag is produced in HEK293 Cells and the target gene encoding Leu25-Gln167 is expressed with a 6His tag at the C-terminus.

Size

50ug,100ug,500ug

Purification

Affinity Purification

Purity

>90%-95% as determined by SDS-PAGE

Endotoxin

<1.0 EU/ug determined by the LAL method

Buffer

20mM Tris, 150mM NaCl, pH8.0

Storage

For long term storage, store the reagent at -80°C. Avoid repeated freeze-thaw cycles.

Shipping Condition

Lyophilized powder is shipped at ambient temperature. Liquid form is shipped in dry ice.

Note

This product is for research use only. This product can not be used for human.

Reference

1 Francisco, L. M., Sage, P. T., and Sharpe, A. H., Immunological Reviews, 2010, DOI: 10.1111/j.1600-065X.2010.00923.x

2 Keir, M. E., Francisco, L. M., and Sharpe, A. H., Annual Review of Immunology, 2007, DOI: 10.1146/annurev.immunol.26.021607.090331

3 Pardoll, D. M., Nature Reviews Cancer, 2012, DOI: 10.1038/nrc3239

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