scFv Antibody Discovery Service

Alpha Lifetech Inc. is proud to offer comprehensive scFv library construction services to meet the diverse needs of global scientists. Our aim is to understand and meet the demands of different customers and to assist with any upcoming and emerging problems in research work.

What Is ScFv- Single Chain Variable Fragment Antibody?

A single-chain antibody consists of an antibody variable light (VL) and heavy (VH) fragment joined by a flexible peptide linker, which can be easily expressed in a functional form on the surface of phage and is therefore commonly known as a single-chain variable fragment (scFv). scFv fragment is the smallest unit of immunoglobulin molecule (except nanobodies) with function in antigen-binding activities and allows protein engineering to improve the properties of scFv (single chain fragment variable) such as increase of affinity and alteration of specificity. In Alpha Lifetech Inc., scFv antibodies have been constructed mainly from hybridoma, spleen cells from immunized mice, and B lymphocytes from humans. Total RNA or mRNA is first isolated from hybridoma, spleen, lymph cells, and bone marrow cells followed by reverse transcribed into cDNA to serve as a template for antibody gene amplification (PCR).

ScFv Antibody Development-Phage Display Technology

The diversity of a library is determined by the number of independent clones. To produce a large-sized scFv antibody library, our scientists will utilize our proprietary two-step strategy for library generation. In the first step of the procedure described, primary repertoires are prepared from PCR products encoding the VH, Vκ, and Vλ domains, yielding typical medium-sized libraries (1 - 100 million clones). In the second step, VH (or VL) fragments are isolated by digestion of plasmid DNA purified from the primary repertoires and cloned into the acceptor phagemid vector containing the VL (or VH) repertoires. This innovation increases the size of the libraries dramatically (1010-1012 clones).

Large-scale and high throughput Screening of scFv Antibody

For large-scale preparation of scFv, it is evident that the choice of expression host will have a significant impact on process design, downstream processing strategies, and process cost. The choice of manufacturing route will be dependent on the relevance of post-translational modification and timeline for clinical development and economics, which are also essentially 'host system '-dependent. There is no universal expression system that ensures high product expression levels for a broad range of different antibodies or even proteins.

Recommend Service

● scFv Antibody Library Construction Service

● scFv Antibody Library Screening Service

● scFv Antibody Expression Service

● scFv Antibody Purification Service

● scFv Antibody Characterization and Functional Validation Services

● scFv Antibody Humanization Services

If you have any questions, please feel free to contact us at any time.